qPCRBIO SyGreen 1-Step Kits

qPCRBIO SyGreen 1-Step Kits

qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube. 
 
Select from qPCRBIO SyGreen 1-Step Detect for the detection of extremely low copy number targets, or qPCRBIO SyGreen 1-Step Go for rapid and accurate results from high RNA template concentrations. 
 
Features
  • Thermostable reverse transcriptase 45°C to 55°C 
  • Advanced RNase inhibitor 
  • Non-PCR inhibiting intercalating dye 
  • Rapid extension rate for early Ct values 
  • Market-leading sensitivity 
  • Increased limit of detection 
  • Antibody-mediated hot start PCR 
  • Compatible on all standard and fast cycling real-time PCR platforms 
Applications
  • Absolute quantification
  • Relative gene expression analysis
  • Detection of extremely low copy number targets
  • qPCRBIO SyGreen 1-Step Detect recommended for template amounts of 1pg - 10ng total RNA or >0.01pg mRNA per reaction
  • qPCRBIO SyGreen 1-Step Go recommended for template amounts of 10pg - 100ng total RNA or >0.01pg mRNA per reaction