qPCRBIO SyGreen 1-Step Kits
qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube.
Select from qPCRBIO SyGreen 1-Step Detect for the detection of extremely low copy number targets, or qPCRBIO SyGreen 1-Step Go for rapid and accurate results from high RNA template concentrations.
Features
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Thermostable reverse transcriptase 45°C to 55°C
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Advanced RNase inhibitor
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Non-PCR inhibiting intercalating dye
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Rapid extension rate for early Ct values
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Market-leading sensitivity
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Increased limit of detection
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Antibody-mediated hot start PCR
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Compatible on all standard and fast cycling real-time PCR platforms
Applications
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Absolute quantification
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Relative gene expression analysis
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Detection of extremely low copy number targets
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qPCRBIO SyGreen 1-Step Detect recommended for template amounts of 1pg - 10ng total RNA or >0.01pg mRNA per reaction
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qPCRBIO SyGreen 1-Step Go recommended for template amounts of 10pg - 100ng total RNA or >0.01pg mRNA per reaction