DASbox® Mini Bioreactor

DASbox® Mini Bioreactor

With small working volumes, compact design and advanced control and information management options the Eppendorf DASbox Mini Bioreactor System addresses demands in research and early process development. Combined with the DASware Software Suite it is an optimum tool for Design of Experiments (DoE) approaches as well as standardising and streamlining process development.

Product Information

The DASbox is a unique mini bioreactor system suitable for microbial and cell culture as well as stem cell applications. It is designed as a 4-fold system with up to twenty-four parallel operating bioreactors. With working volumes of 60 – 250 mL the DASbox is the optimal tool for advanced process development and Design of Experiments (DoE) applications. All critical process parameters can be precisely controlled. Liquid-free temperature control and exhaust condensation satisfy users with easy handling.In addition to using industry standard glass bioreactors the DASbox can be equipped with Eppendorf BioBLU 0.3 vessels, all fully instrumented single-use mini bioreactors.


  • Parallel set-up of up to 24 bioreactors
  • Excellent scalability and reproducibility in both microbial and cell culture applications
  • Supports industry standard glass bioreactors (DASbox Mini Bioreactor) as well as BioBLU 0.3 Single-Use Vessels
  • Small working volumes save on the amount of cell material, media and supplements required
  • Extremely compact system with a footprint of only 7.5 cm (3 in) benchspace per vessel
  • Individual temperature control with liquid-free heating and cooling (Peltier)
  • Liquid-free Peltier exhaust condenser with easy handling by automatic slide in activation and slide out deactivation mode
  • LC display with key process parameters and integrated alarm function simplifies monitoring
  • Fully mass flow-controlled gas mixing with individual gas mixture from air, N2, O2, and CO2, each directable either to headspace or sparger
  • Standard sensors for precise measurement and control of temperature, pH, DO, level and ORP (redox potential); optical pH sensors available
  • Precise miniature variable speed pumps, continuous flow rates down to 0.3 mL/h
  • Sealed magnetic overhead drives for single-use vessels and direct overhead drives for autoclavable vessels; up- or downflow selectable
  • Optional pull-out system for enhanced accessibility of bioreactors and control unit


  • Process development in cell culture and microbiology
  • Controlled cultivation of stem cells
  • Design of Experiments (DoE)
  • Media optimization
  • Clone and cell line screening, strain characterization