Agency
UltraScript Reverse Transcriptase
UltraScript Reverse Transcriptase is a robust and thermostable modified MMLV reverse transcriptase engineered to enhance cDNA synthesis speed and yield with accurate transcript representation.
The enzyme allows highly sensitive reverse transcription of viral RNA sequences, making it an ideal component of RT-qPCR-based SARS-CoV-2 detection tests.
Features
-
Recommended product for reverse transcription step of COVID-19 diagnostic tests
-
Sensitive and accurate reverse transcription of viral RNA sequences, including SARS-CoV-2
-
Thermostable reverse transcriptase 45°C to 55°C
-
Advanced RNase inhibitor
-
High cDNA yields from as little as 4pg total RNA
-
Accurate reverse transcription of GC-rich templates
-
Sensitive detection of low copy number transcripts
-
Advanced buffer chemistry including Mg and dNTPs
Applications
-
Random hexamer, oligo(dT) and gene-specific primers
-
cDNA synthesis for PCR analysis, cloning, library preparation and Next Generation Sequencing
-
Low copy number transcripts
-
Viral RNA targets
-
miRNA targets
-
Efficient synthesis from total RNA or poly(A)+ RNA