qPCRBIO cDNA Synthesis Kit

qPCRBIO cDNA Synthesis Kit

High quality cDNA synthesis is essential for downstream real-time PCR analysis and successful expression studies. qPCRBIO cDNA Synthesis Kit is an easy-to-use 2 tube system specifically developed to generate cDNA for use in real-time PCR. 
 
The reverse transcriptase, buffer system and optimised blend of random hexamers with anchored oligo(dT) primers provide unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. 
 
Features
  • Unbiased representation of 5’ and 3’ mRNA transcript ends
  • Sensitive detection of low copy number transcripts
  • High cDNA yields from as little as 4pg total RNA
  • Reduced RNase H activity 
  • Simple 2 tube system
  • 5x buffer contains anchored oligo(dT), random hexamers, enhancers, dNTPs and MgCl2
  • 20x thermostable reverse transcriptase blended with RNase inhibitor
Applications
  • cDNA synthesis for real-time PCR analysis
  • Low copy number transcripts
  • Viral RNA targets
  • Efficient synthesis from total RNA or poly(A)+ RNA